Even `bowtie2`’s `–very-sensitive-local` option can be a little grumpy. Here’s how I make `bowtie2` a little more relaxed for alignment of more dissimilar sequences, particularly for my work on metagenomes.
This is the third occasion that I’ve come to set up a Python package’s documentation. It is also the third occasion where I’ve spent many hours and commits trying to work out how I made everything work last time. So for my future sanity and possibly yours, here’s a quick guide on setting up Sphinx-based, ReadTheDocs-hosted Python documentation.
Earlier this afternoon, my server was upset. Here’s what happened, how it took so long to find out, and how I will try and stop it happening again. In particular, I provide configuration for a `fail2ban` jail to try and stop simple DOS, that actually works.